Gene transfer by electroporation, lipofection, and DEAE-dextran transfection: Compatibility with cell-sorting by flow cytometry,

DEAE-dextran enhances electroporation of mammalian cells.

Electroporation has proven to be a convenient method for transfection of many cell types. One drawback has been that it requires large amounts of DNA, typically 20 to 80 /tg per transfection (1). Here we report that adding DEAE-dextran to the electroporation media dramatically increases electroporation efficiency in mammalian cells, lowering the amount of DNA required for efficient transfection...

The kinetics of DEAE-dextran-induced cell sensitization to transfection.

non-viral transfection methods optimized for gene delivery to a lung cancer cell line

background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...

Non-Viral Transfection Methods Optimized for Gene Delivery to a Lung Cancer Cell Line

BACKGROUND Mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. This study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection Procedures. METHODS In the current study, calcium phosphate (CaP), DEAE-...

Inhibition of growth of human prostate cancer xenograft by transfection of p53 gene: gene transfer by electroporation.

To date, there is no effective therapy for hormone-independent prostate cancer. Therefore, as a new strategy for refractory cancer, gene therapy is showing increasing promise. In this study, we attempted to use a nonviral gene transfer system, in vivo electroporation, in prostate cancer cell PC-3 xenografts with the wild-type p53 (wt-p53) gene, as gene therapy for hormone-independent prostate c...